Comments for binary|fusion http://adamcoster.com ramblings of a fetal biologist Thu, 19 Apr 2012 13:00:34 +0000 hourly 1 http://wordpress.com/ Comment on Problem solved! by adamcoster http://adamcoster.com/2008/04/30/problem-solved/#comment-1059 Thu, 19 Apr 2012 13:00:34 +0000 http://adamcoster.wordpress.com/?p=105#comment-1059 Crap, I wasn’t aware that my reference link went down. I used the “wayback machine” to find an archived version of the page, which you can find here:

http://web.archive.org/web/20110514130212/http://diveintomark.org/archives/2007/02/05/authentication-failed-during-upgrade

I hope that helps, because otherwise I have no idea!

]]> Comment on Problem solved! by anum http://adamcoster.com/2008/04/30/problem-solved/#comment-1058 Thu, 19 Apr 2012 12:43:37 +0000 http://adamcoster.wordpress.com/?p=105#comment-1058 hey im having the same problem with my ubuntu upgrade and i did what you suggested here but nothing happened and the problem is still there. can you suggest something else?

]]> Comment on The universe is silent and colorless by Jonah http://adamcoster.com/2008/04/22/universe-silent-colorless/#comment-1051 Sat, 07 Apr 2012 17:02:09 +0000 http://adamcoster.wordpress.com/?p=101#comment-1051 There’s a saying in the tradition of Vedanta:

If you can see it,
touch it,
taste it,
smell it,
imagine it,
conceptualize it,
visualize it,

then, it’s not real.

]]> Comment on cloning trick: ligation of multiple inserts by adamcoster http://adamcoster.com/2011/07/11/cloning-trick-ligation-of-multiple-inserts/#comment-1044 Tue, 27 Mar 2012 03:16:28 +0000 http://adamcoster.com/?p=1184#comment-1044 I cheated by using a really good polymerase :) . Then I sequenced a few clones to be sure.

]]> Comment on cloning trick: ligation of multiple inserts by Robert http://adamcoster.com/2011/07/11/cloning-trick-ligation-of-multiple-inserts/#comment-1043 Tue, 27 Mar 2012 02:27:42 +0000 http://adamcoster.com/?p=1184#comment-1043 The only problem with this apporach is that the PCR step could introduce errors into your product which could or could not be very bad (lets say a stop codon in your marker gene or gene of interest). You seem pretty savey though so you probably calculated the probability of an error based off the fidelity of your polymerase!

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